Isolation and identification of N⁶-isopentenyladenosine as the cytotoxic constituent of a marine sponge Oceanapia sp.

ABSTRACT

N⁶-Isopentenyladenosine (i⁶A) was isolated from a marine sponge Oceanapia sp. as the major cytotoxic constituent along with N⁶-isopentenyladenosine 5ʹ-monophosphate (i⁶AP) which was inactive. The structures of i⁶A and i⁶AP were assigned by a combination of the analysis of NMR spectroscopy and mass spectrometry. This is the first isolation of i⁶A and i⁶AP from a marine sponge.

KEYWORDS:

• Marine sponge
• N⁶-isopentenyladenosine
• N⁶-isopentenyladenosine 5ʹ-monophosphate
• cytotoxicity
• cytokinin

Acknowledgments

We thank the captain and crew of the T/S Toyoshio-maru for sample collection.

Author contribution

SN isolated and identified the compounds and wrote the draft of the manuscript. YI identified the sponge. SOh conducted the collection of the sponge. SOk participated to design the study. SM designed the study and prepared the manuscript. All authors reviewed and approved the final manuscript.

Disclosure statement

No potential conflict of interest was reported by the authors.

Supplementary material

Supplemental data for this article can be accessed here.

Additional information

Funding

This work was supported by JSPS KAKENHI 17H06403; Japan Society for the Promotion of Science [KAKENHI 17H06403].