Preparation and evaluation of stingray skin collagen/oyster osteoinductive composite scaffolds

Abstract

The regeneration of bone defects is a major challenge for clinical orthopaedics. Herein, we designed and prepared a new type of bioactive material, using stingray skin collagen and oyster shell powder (OSP) as raw materials. A stingray skin collagen/oyster osteoinductive composite scaffold (Col-OSP) was prepared for the first time by genipin cross-linking, pore-forming and freeze-drying methods. These scaffolds were characterized by ATR-FTIR, SEM, compression, swelling, cell proliferation, cell adhesion, alkaline phosphatase activity, alizarin red staining and RT-PCR etc. The Col-OSP scaffold had an interconnected three-dimensional porous structure, and the mechanical properties of the Col-OSP composite scaffold were enhanced compared with Col, combining with the appropriate swelling rate and degradation rate, the scaffold was more in line with the requirements of bone tissue engineering scaffolds. The Col-OSP scaffold was non-toxic, promoted the proliferation, adhesion, and differentiation of MC3T3-E1 cells, and stimulated the osteogenesis-related genes expressions of osteocalcin (OCN), collagen type I (COL-I) and RUNX2 of MC3T3-E1 cells.

Keywords:

• stingray skin collagen
• oyster shell powder
• composite scaffold
• bone repair

Author contributions

Yue Wu: Investigation, Visualization, Data curation, Writing-Reviewing and Editing. Yingkun Fu: Visualization, Investigation. Hongfu Pan: Visualization, Investigation. Cong Chang: Visualization, Investigation. Ningjian Ao: Visualization, Investigation. Hui Xu: Visualization, Investigation. Zhengnan Zhang: Software, Validation. Ping Hu: Software, Validation. Riwang Li: Software, Validation. Shuxia Duan: Supervision, Project administration. Yan Yan Li: Conceptualization, Methodology, Writing-Reviewing and Editing.

Disclosure statement

No potential conflict of interest was reported by the authors.

Ethical approval

The animal experiments were completed according to the protocols approved by the Laboratory Animal Center, Jinan University, Guangzhou, China. All animal care and treatment were approved by the Laboratory Animal Care Agency.

Additional information

Funding

The authors gratefully acknowledge the support of the “National Natural Science Foundation of China” for this study (Award Number: 20976068). This work was supported by the Science and Technology Department project of Guangdong Province (2017A020211006), the Science and Technology Department project of Henan Province (201111310200).