In Vivo Confocal Microscopy in Blepharitis Patients with Ocular Demodex Infestation

ABSTRACT

Purpose

To compare corneal in-vivo confocal microscopy (IVCM) findings in patients with seborrheic blepharitis with healthy controls and to evaluate the correlation with the Demodex load.

Methods

Sixty-two Demodex positive seborrheic blepharitis patients and 24 controls were included. Four eyelashes from each eye were examined. The median value of Demodex count (5 mites/eye) was assigned as the cutoff value to create low and high Demodex infestation subgroups. Corneal nerve parameters (fiber length;CNFL, fiber density;CNFD, branch length;CNBL, branch density;CNBD) and dendritic cell density (DCD) were analyzed by IVCM for all participants.

Results

The mean DCD of eyes in low (37.62 ± 25.18 cells/mm²) and high (76.00 ± 44.00 cells/mm²) Demodex load groups were significantly higher than those in the control group (14.25 ± 2.68 cells/mm²) (p < .001). CNFL, CNFD, CNBL and CNBD were significantly lower in low and high Demodex groups compared to controls (p < .001).

Conclusions

Demodex positive seborrheic blepharitis patients showed significantly reduced density of corneal nerves and elevated DCD levels.

KEYWORDS:

• Confocal microscopy
• Demodex
• dendritic cell density
• seborrheic blepharitis
• sub-basal nerve plexus

Declaration of interest

The authors report no conflicts of interest. The authors alone are responsible for the content and writing of the paper.