Attenuation of hepatocellular carcinoma by naringenin is associated with modulation of circadian rhythms of redox status in rats

Abstract

We evaluated the effect of naringenin on the circadian rhythms of circulatory lipid peroxidation and antioxidant status during N-Nitrosodiethylamine (NDEA)-induced hepatocarcinogenesis in rats. The characteristics of circadian rhythms (acrophase, amplitude and mesor) of lipid peroxidation products thiobarbituric acid reactive substances (TBARS) and antioxidant enzymes (superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GPx) and glutathione-S-transferase (GST)) were markedly altered in NDEA-treated rats when compared to other groups. Pre- and post-treatment of naringenin to NDEA treated animals significantly increased the mesor and alter amplitude values of antioxidants and significantly decreased the mesor values of TBARS. Further delays in acrophase in NDEA-treated rats were reversed by naringenin administration. Our findings suggest that naringenin could alter the circadian characteristics of lipid peroxidation and antioxidant status, and further research is in process on the chronotherapeutic efficacy of naringenin.

Keywords:

• Circadian rhythm
• hepatocellular carcinoma
• lipid peroxidation
• antioxidants
• naringenin