Role of macrophage colony-stimulating factor (M-CSF) in human granulosa cells

Abstract

Macrophage colony-stimulating factor (M-CSF) has been proved to have a positive role in the follicular development. We investigated its effect on human granulosa cells and found that M-CSF could stimulate the production of E₂. The production of FSH receptors was enhanced by M-CSF in vitro in a dose-dependent manner with or without the addition of tamoxifen (p <0.05). Correspondingly, FSH was also able to coordinate the expression of M-CSF and its receptor (p <0.05). That maybe important to maintain the level of Nppc and the meiotic arrest of the oocyte. The protein p-JAK2 and p-STAT3 in JAK/STAT-signaling pathway elevated after the influence of M-CSF (p < 0.05). These results suggest that M-CSF has a role in regulating the response of granulosa cells to gonadotropins. Its function is associated with JAK/STAT-signaling pathway.

Chinese abstract

巨噬细胞集落刺激因子 (M-CFS) 已被证实在卵泡发育过程中起着积极的作用。我们研究M-CFS对人类卵巢颗粒细胞的影响发现它可以刺激雌二醇的生成。在体外实验中发现无论是否添加他莫昔芬, M-CFS以剂量依赖的方式提高卵泡刺激素 (FSH) 受体的生成 (P＜0.05) , 相对地, FSH也可以协调M-CSF及其受体的表达 (P＜0.05) 。这可能对于卵母细胞保持C-型钠肽 (Nppc) 的水平和阻止减数分裂很重要。M-CSF会提高JAK/STAT信号转导通路中 p-JAK2 和 p-STAT3 蛋白的表达 (P＜0.05) 。这些结果提示M-CSF可以调节卵巢颗粒细胞对促性腺激素的反应。这个功能与JAK/STAT信号转导通路有关。

Keywords:

• Estrogen
• FSH
• granulosa cell
• JAK/STAT pathway
• M-CSF
• tamoxifen

Declaration of interest

The authors declare that they have no competing interests.

The study was funded by the Science Foundation of Zhejiang Province [NO:Y2090796].