Serum substance P concentrations to predict oocyte maturation index and clinical pregnancy

Abstract

Aim: The aim of this study was to assess the predictive value of serum substance P (SP) concentrations on oocyte maturation and clinical pregnancy.

Methods: Ninety-three women with unexplained infertility underwent intracytoplasmic sperm injection (ICSI) cycles. Antagonist protocol was started for each participant and at the day of oocyte pick up, serum samples were obtained from each participant to assess SP concentrations, and these concentrations were utilized to predict mature/total oocyte ratio and clinical pregnancy.

Results: SP concentration was a significant predictor for mature/total oocyte ratio > 0.75 and clinical pregnancy. In correlation analyses, maturation index was significantly correlated with FSH (r= −0.226, p = 0.03), estradiol (r = 0.239, p = 0.021), peak estradiol (r = 0.414, p < 0.001), and substance P (r = 0.796, p < 0.001). In multivariate analyses, number of immature (beta coefficient = −0.379, p < 0.001), mature oocyte (beta coefficient = 0.473, p < 0.001), SP concentration (beta coefficient = 0.723, p < 0.001) and maturation index (beta coefficient = −0.387, p = 0.003) were significantly associated with clinical pregnancy.

Conclusion: SP concentrations at the day of oocyte pick up may be used to predict clinical pregnancy and may be an indirect indicator for cycle outcome in assisted reproductive technology (ART).

Chinese abstract

目的：本研究的目的为评估血清P物质 (SP) 浓度对卵母细胞成熟和临床妊娠的预测价值。

方法：93名不明原因不孕不育的女性接受了胞浆内单精子注射 (ICSI) 周期。每个受试者在取卵当天开始拮抗剂方案, 并收集受试者的血清样本以评估SP浓度, 同时SP浓度用于预测成熟/总卵母细胞比例和临床妊娠。

结果：SP浓度是成熟/总卵母细胞比例>0.75是临床妊娠的重要预测指标。相关性分析显示, 成熟指数与FSH(r=-0.226,p = 0.03), 雌二醇(r = 0.239,p = 0.021), 峰值雌二醇(r = 0.414,p＜0.001), P物质显著相关。多元分析显示, 未成熟卵母细胞数 (β系数=-0.379,p＜0.001), 成熟卵母细胞数(β系数=0.473,p＜0.001), SP浓度(β系数=0.723,p＜0.001)与成熟指数(β系数=-0.387, p = 0.003)与临床妊娠显著相关。

结论：取卵当日的SP浓度或可用于预测临床妊娠, 并可作为辅助生殖技术(ART)中周期结局的间接指标。

Keywords:

• Assisted reproduction
• implantation
• maturation index
• substance P

Declaration of interest

Authors have nothing to declare.