Abstract
The purpose was to investigate the correlation between pronuclei (PN) morphology and morphokinetic behaviors of derived embryos with time lapse monitoring (TLM) in assisted reproduction setting. Over time, PN morphology from PN appearance (PNA) to PN fading (PNF), PNF according to size, contact, number and position of nuclear precursor bodies (NPBs) within each PN and morphokinetics variables, including absolute time points, relative timing parameters, cleavage patterns and arrest rate, were evaluated using TLM. There were insignificant relationship between morphokinetics variables including tBP2, tPNA, tPNF, t2, t3, t4, t5, t6, t7, t8, S1, CC2, S2 and Z scoring according Z1 to Z4 (p > .05). Also, an insignificant relationship was noticed between uneven blastomeres, reverse cleavage embryos and Z scoring (p > .05). However, there were significant correlations between the rates of direct and arbitrary cleavage as well as arrested embryos and Z scores. Combined PN morphology and embryo kinetic evaluation were suggested in assisted reproduction programs.
Chinese abstract
本文旨在对辅助生殖技术中胚胎原核(PN)形态和延迟摄像(TLM)监测的胚胎形态动力学特征之间的相关性进行研究。使用TLM评估从PN出现(PNA)到PN凋亡(PNF)的PN形态, 参考的指标包括每个PN内的核仁前体(NPBs)的大小、致密度、数量和位置以及形态动力学变量(包括绝对时间点, 相对定时参数, 卵裂模式和停育率)。形态动力学变量中tBP2, tPNA, tPNF, t2, t3, t4, t5, t6, t7, t8, S1, CC2, S2和Z评分(Z1到Z4)之间没有显著的相关性(p > 0.05)。 另外, 在不均匀的卵裂球、反向卵裂胚胎和Z评分之间的关系也不明显(p > 0.05)。 然而, 直接和任意卵裂率以及停育胚胎和Z评分之间存在显着的相关性。 在辅助生殖胚胎评分中建议用PN形态学和胚胎动力学联合评价。
Disclosure statement
The authors report no conflicts of interest. The authors alone are responsible for the content and writing of this article.