Gene expression changes in arterial and venous endothelial cells exposed to gestational diabetes mellitus

Abstract

We investigated the molecular changes in fetoplacental blood vessel endothelial cells in gestational diabetes mellitus (GDM). Raw gene expression profile data of arterial and venous endothelial cells from GDM complicated pregnancies and healthy controls were downloaded and used for bioinformatic analysis. There were two differentially expressed genes (DEGs) in venous endothelial cells and 178 DEGs in arterial endothelial cells induced by GDM. The altered genes were clustered to pathways associated with cell cycle, p53 signaling pathway, and cellular senescence. The disease associated gene-pathway network that was constructed comprised eight down-regulated genes (including FBXO5, CCNB1, and CDK1), one up-regulated gene (CCND2), hsa04068: FoxO signaling pathway and hsa04114: Oocyte mitosis pathway. CCND2 was a significant node in the microRNA (miRNA)-target network, which was regulated by seven miRNAs that included hsa-miR-1299, hsa-miR-1200, and hsa-miR-miR-593-5p. FBXO5 was a significant node regulated by two miRNAs. CCND2 and FBXO5 were also the significant nodes in the transcriptional factors-target network and integrated regulatory network. The cell cycle pathway was significantly altered in arterial endothelial cells during GDM, which was involved with the differential expression of CCND2 and FBXO5.

摘要

我们研究了妊娠糖尿病（GDM）孕妇胎儿血管内皮细胞的分子变化。下载GDM合并妊娠和健康对照的动脉和静脉内皮细胞的原始基因表达谱数据, 用于生物信息学分析。暴露于GDM胎儿静脉内皮细胞有2个差异表达基因, 动脉内皮细胞有178个差异表达基因。差异基因聚集在与细胞周期、p53信号通路和细胞衰老相关的通路上。构建的疾病相关基因通路网络由8个下调基因（包括FBXO5、CCNB1、CDK1）、1个上调基因（CCND2）、hsa04068：FoxO信号通路和hsa04114：卵母细胞有丝分裂通路组成。CCND2是microRNA (miRNA)靶点网络中的重要节点, 该网络受包括hsa-miR-1299、hsa-miR-1200和hsa-miR-miR-593-5p在内的7个miRNA调控。FBXO5是一个受两种mirna调控的重要节点。CCND2和FBXO5也是转录因子靶网络和综合调控网络中的重要节点。GDM患者动脉内皮细胞的细胞周期通路发生显著改变, 这与CCND2和FBXO5的差异表达有关。

The Chinese abstracts are translated by Prof. Dr. Xiangyan Ruan and her team: Beijing Obstetrics and Gynecology Hospital, Capital Medical University, Beijing 100026, China.

Keywords:

• Gestational diabetes mellitus
• pathway
• differentially expressed gene
• miRNA
• transcription factor

Disclosure statement

The authors report no conflicts of interest.

Data availability statement

The data used to support the findings of this study are available from the corresponding author upon request.