Variation analysis of Ghrelin gene in Chinese patients with obesity, having polycystic ovarian syndrome

Abstract

Association of single-nucleotide polymorphisms (SNPs) of the ghrelin gene with polycystic ovary syndrome (PCOS)is unclear. However, their correlation with PCOS-related obesity has been observed. The objective of this study was to evaluate the effects of ghrelin gene SNPs on PCOS-related obesity in Chinese women. The full-length sequence of the ghrelin gene was determined to explore the relationship of the SNPs with PCOS-related obesity in Chinese women. The gene was sequenced, including all exons, introns and exon–intron boundaries in 230 Han Chinese women with PCOS and 162 normal women. Significant genotypic and allelic differences were observed between the obese PCOS group and obese control group at rs35681 locus (p = .013 and .017). The genotypic analysis of obese and non-obese people in the PCOS group showed that the proportion of A allele in the obese PCOS group (10.9%) was higher than that of the G allele (3.6%). This study revealed that ghrelin rs35681 might be related to the occurrence of obesity associated with PCOS, and allele A was found to increase the risk of obesity in PCOS.

摘要

Ghrelin基因单核苷酸多态性（SNPs）与多囊卵巢综合征（PCOS）的关系尚不清楚。然而, 已经观察到了Ghrelin基因单核苷酸多态性与PCOS相关的肥胖之间的相关性。本研究的目的旨在评估ghrelin基因SNPs在中国女性多囊卵巢综合征相关肥胖中的作用。测定ghrelin基因序列全长, 探讨SNPs与中国女性PCOS相关肥胖的关系。对230例中国汉族多囊卵巢综合征女性和162例正常女性进行了基因测序, 包括所有外显子、内含子和外显子-内含子边界序列。在rs35681位点观察到PCOS肥胖组和肥胖对照组之间基因型和等位基因存在显著差异(p＝0.013和0.017)。PCOS组中肥胖者与非肥胖者基因型分析显示, 肥胖型PCOS组A等位基因比例(10.9%)高于G等位基因比例(3.6%)。本研究显示ghrelin rs35681可能与PCOS相关肥胖的发生有关, 并且发现等位基因A增加了PCOS患者肥胖的风险。

The Chinese abstracts are translated by Prof. Dr. Xiangyan Ruan and her team: Beijing Obstetrics and Gynecology Hospital, Capital Medical University, Beijing 100026, China.

Keywords:

• Ghrelin
• obesity
• polycystic ovary syndrome
• single-nucleotide polymorphism

Acknowledgements

The authors appreciate the patients for their participation.

Disclosure statement

The authors declare that they have no conflict of interest.

Additional information

Funding

This study was supported by grants from the National Natural Science Foundation of China [grant number 81571408].