The interchangeability of two assays for the measurement of anti-Müllerian hormone when personalizing the dose of FSH in in-vitro fertilization cycles

Abstract

Objective

Study the interchangeability of Roche Elecsys and Beckman Coulter Access anti-Müllerian Hormone (AMH) assays to select the gonadotropin starting dose in IVF cycles.

Methods

Patients’ AMH was measured using both Elecsys and Access automated assays. AMH values were then used to calculate the FSH starting dose. The main outcome is the percentage of women that would have been stratified to a different dose of gonadotropin due to differences in AMH values from the two tests.

Results

The Access assay systematically gives higher values compared with the Elecsys assay (slope = 0.88). For Follitropin Alfa, the difference in starting dose was > 15% in 2/113 patients, when Access AMH was used instead of Elecsys. For Follitropin Delta the difference in the starting dose was >15% in 21/113 patients when using Access AMH. When considering women with high ovarian reserve, only 4/51 would have received a Follitropin Delta dose that exceeded a 15% difference using Access AMH as a substitute for the Elecsys value.

Conclusions

The use of the Roche Elecsys or Beckman Coulter Access leads to modest differences in AMH values, which seem to little affect the calibration of FSH dose used for ovarian stimulation.

摘要

目的：研究罗氏Elecsys和贝克曼-库尔特Access两种检测抗缪勒氏管激素(AMH)方法的互换性, 以选择体外受精(IVF)周期中促性腺激素的起始剂量。

方法：采用Elecsys和Access两种自动分析方法测定患者的AMH。然后用AMH值计算FSH起始剂量。主要结局是由于两种检测方法的AMH值不同而被分层至不同剂量促性腺激素的女性的百分比。

结果：Access测定法系统地给出了比Electrsys测定法更高的值(斜率＝0.88)。对于Follitropin Alfa, 当使用Access AMH而不是Elecsys时, 有2/113的患者的起始剂量差异>15%。对于Follitropin Delta, 当使用Access AMH时, 有21/113的患者的起始剂量差异>15%。在卵巢储备较高的女性中, 使用Access AMH替代Elecsys, 只有4/51的患者其Follitropin Delta剂量差异超过15%。

结论：使用罗氏Electrsys或Beckman Coulter Access会导致AMH值的轻微差异, 这似乎对用于卵巢刺激的FSH剂量的校准几乎没有影响。

Keywords:

• Anti-müllerian hormone
• assay
• Follitropin Delta
• nomogram
• ovarian reserve

Disclosure statement

No potential conflict of interest was reported by the author(s).

Additional information

Funding

This work was supported by an unrestricted research grant by the Beckman Coulter USA.