Differential expression of Lumican, Mimecan, Annexin A5 and Serotransferrin in ectopic and matched eutopic endometrium in ovarian endometriosis: a case-control study

Abstract

Aim

Endometriosis is a debilitating disease marked by recurrent gynecological proliferations. The present study aimed at performing differential proteomic analysis of matched eutopic and ectopic endometrium from women with ovarian endometriosis.

Materials and methods

Proteomes were resolved using nano LC–MS and further identified and quantified using ProteinLynx Global SERVER (PLGS) software. Selected proteins were further chosen for validation by real time-polymerase chain reaction (RT-PCR).

Results

The protein profiles uncovered several differentially expressed proteins in the diseased sample (ectopic endometrium) as compared to the reference sample (eutopic endometrium). The study involved an advanced proteomic approach, nano LC–MS, and validates for the first time the upregulation of Mimecan and Lumican proteins in endometriosis.

Conclusions

These proteins may hence prove as potentially useful tools in the search for diagnostic markers for early detection of the disease.

摘要

目的:子宫内膜异位症是一种以复发性子宫内膜异位种植为特征的进行性疾病。本研究旨在对卵巢子宫内膜异位症患者的匹配在位和异位子宫内膜进行差异蛋白质组学分析。材料和方法:使用纳米液相色谱-质谱技术解析蛋白质组, 并使用蛋白Lynx全球服务器(PLGS)软件进一步鉴定和定量。通过实时PCR进一步对选定的蛋白质进行验证。结果:蛋白质谱揭示了与参考样品(在位子宫内膜)相比, 患病样品(异位子宫内膜)中几种差异表达的蛋白质。这项研究涉及一种先进的蛋白质组学方法, 纳米液相色谱质谱, 并首次验证了Mimecan和Lumican蛋白在子宫内膜异位症中的上调。结论:这些蛋白质可能被证明是在寻找子宫内膜异位症早期诊断的检测标记物中潜在有用的工具。

Keywords:

• Ovarian endometriosis
• eutopic
• ectopic
• proteomes
• nano LC–MS
• -PCR

Ethical approval

The experimental plan was approved by the JHIEC. Informed written consent was taken from all the participants.

Author contributions

TS collected samples and clinical details of the subjects, conducted the experiments, and wrote the manuscript. AN is the clinical coordinator of the study. SA and FW and SJ reviewed the manuscript. SW designed the study, provided research facilities, analyzed data and finalized the manuscript.

Disclosure statement

The authors declare no conflict of interest.

Additional information

Funding

The study was supported by University Grants Commission, India by providing fellowship to Tahreem Sahar under UGC NET JRF Scheme [22/12/2013(ii) EU-V].