Preliminary study of Yulin mixture affecting the miR-320/SF-1/Cyp19a1 on mouse polycystic ovary syndrome model

Abstract

In this study, the polycystic ovary syndrome (PCOS) mice model was randomly divided into 6 groups: blank control group, Clomiphene group, PCOS group, and Yulin mixture high-/medium-/low-dose group. Rats were killed after 5 weeks of administration. The expression levels of serum E₂,T,Insulin and LH were detected by ELISA. The localizations and quantities of Steroid-generating factor-1 (SF-1) and Cytochrome protein P450 a1 (Cyp19a1) were detected by immunohistochemistry and western blot. The quantities of miR-320 were detected by RT-PCR. The results showed that the mechanism of Yulin mixture inhibiting the growth of polycystic ovary on mouse PCOS model may be through the decreasing of serum T and LH levels and then reducing local estrogen content to make the polycystic ovary atrophy. Yulin mixture can decrease the level of miR-320 and increase the expression of SF-1 and Cyp19a1 in ovary, thereby regulating the ovarian granulosa cell proliferation and apoptosis.

摘要

本研究将多囊卵巢综合征(PCOS)小鼠模型随机分为6组:空白对照组、克罗米芬组、PCOS组、Yulin合剂高/中/低剂量组。给药5周后处死大鼠。酶联免疫吸附法检测血清E2、T、胰岛素和黄体生成素的表达水平。免疫组化和蛋白质印迹法检测类固醇生成因子-1 (SF-1)和细胞色素P450 a1 (Cyp19a1)的定位和数量。逆转录聚合酶链反应检测miR-320的数量。结果表明, Yulin合剂抑制PCOS小鼠模型多囊卵巢生长的机制可能是通过降低血清睾酮和黄体生成素水平, 进而降低局部雌激素含量, 使多囊卵巢萎缩。Yulin合剂能降低卵巢中miR-320的水平, 增加SF-1和Cyp19a1的表达, 从而调节卵巢颗粒细胞的增殖和凋亡。

Keywords:

• Steroid-generating factor-1 (SF-1)
• cytochrome protein P450 a1 (Cyp19a1)
• miR-320
• polycystic ovary syndrome (PCOS)
• Yulin mixture

Disclosure statement

No potential conflict of interest was reported by the author(s).

Additional information

Funding

This work was supported by Shanghai University of Traditional Chinese Medicine [grant number 2016YSN50]; Shanghai Municipal population and Family Planning commission [grant number ZY3-JSFC-2-2019]; and Hospital level [grant number 2019YYQ01]. The funders provide only financial aid, and there was no individuals employed or contracted by the funders. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.