Effect of concentrated growth factors on function and Wnt3a expression of human periodontal ligament cells in vitro

Abstract

Concentrated growth factors (CGFs), the new generation of platelet concentrate products, appear to exhibit superior potential for tissue regeneration. However, there are only a few studies supporting this. This study was designed to investigate the effect of CGFs on proliferation and alkaline phosphatase (ALP) activity of human periodontal ligament cells (hPDLCs) in vitro. Furthermore, as bone homeostasis is fundamentally controlled by Wnt-mediated signals, we also investigated Wnt3a expression of hPDLCs after treatment of CGFs. hPDLCs and CGFs were obtained from the same volunteer. CGFs or combination of recombined human TGF-β1 (rhTGF-β1) and PDGF-AB (rhPDGF-AB) were added to hPDLCs in different concentrations. The rate of proliferation was analyzed by an MTT assay. ALP activity was assessed using p-NPP assay. Quantitative RT-PCR was used to evaluate the gene expression of Wnt3a. In a range of concentrations, CGFs significantly promoted the proliferation of hPDLCs in a dose-dependent manner. ALP activity was also enhanced by CGFs in a dose-dependent and time-dependent manner. The stimulatory effect of CGFs was much greater than rhTGF-β1 and rhPDGF-AB combination. Quantitative RT-PCR results showed that Wnt3a mRNA expression was increased at 24 h in hPDLCs treated by CGFs. CGFs can enhance hPDLCs proliferation and ALP activity and may have great potential in clinical and biotechnological applications.

Keywords:

• Alkaline phosphatase activity
• concentrated growth factors
• cell proliferation
• periodontal ligament cells
• Wnt3a

Acknowledgments

The authors would like to thank the members of the Department of Periodontology, the First Outpatient Center, Peking University School, and Hospital of Stomatology for their help in the current investigation.

Declaration of interest statement

The authors declare that there are no conflicts of interest in this study. This work was supported by Ministry of Science and Technology of China under contract International Science & Technology Cooperation Program foundation Nr.1019.