Abstract
A monoclonal antibody-based ELISA was developed for fenbendazole, a widely used benzimidazole anthelmintic, with approved uses in cattle and other food animals. The antibody was elicited using as hapten 2-succinamido-5(6)-phenylthiobenzimidazole, which was conjugated with bovine serum albumin to produce an immunogen and with horseradish peroxidase to prepare a labeled ligand. The ELISA was performed on aqueous extracts of bovine liver or diluted milk samples. In bovine liver, the limit of detection was 200 ppb; in the milk matrix, the limit of detection was 3 ppb. The ELISA method is a simple approach to screen food samples for residues of fenbendazole.