In vitro effect of Pleurotusflorida on macrophage cell viability and nitric oxide production

Abstract

Pleurotus florida is an edible mushroom with various therapeutic uses. Recently, it has been shown to modulate the immune responses and inflammation. Macrophages have been shown to play an essential role as the first line of defence against microbial invaders and neoplasia and have intracellular killing capacities. After being stimulated, macrophages produce a number of inflammatory mediators. In this study, we evaluated the effect of P. florida on viability of macrophages and its nitric oxide (NO) production.

Cell viability of macrophages was altered by extract and fractions dose, dependently. R100, R50 and R30 fractions in some dilutions decreased and extract R10, R5 and F5 fractions increased cell viability. R50, R30, R10, R5 and F5 fractions cause significant reduction and R100 causes significant induction in NO production. Further studies are necessary to clarify the modulatory effects of P. florida mushroom on macrophage anti-inflammatory function.

Keywords:

• Pleurotus florida
• mushroom
• immunomodulator
• cell viability
• macrophage
• NO production

Acknowledgements

This study was performed by Immunoregulation Research Center of Shahed University, and was supported by Medical Research Center, Shahed University.