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Abstract
With rabbit anti-bovine IgG, a reliable, highly selective and cheap method has been optimised and established for the determination of IgG in bovine colostrum. The method was based on the soluble immune complex precipitating from the liquid in the function of polyethylene glycol. Subsequently, the interference of casein, β-lactglobulin, α-lactalbumin, lactofemin and inactive IgG to the immunoassay was investigated. Finally, the assay was applied to the analysis of IgG in spiked milk samples. The limit of detection, calculated as three times of determinations’ standard deviation, was 0.07 mg/mL, with a practical working range between 0.2 and 0.8 mg/mL. The average recoveries of IgG in milk were 97.63%, 99.87%, 100.1% for 0.2 mg/mL, 0.5 mg/mL, 0.8 mg/mL, respectively. Eventually, confirmation test between immunoturbidimetry method and high performance liquid chromatography (HPLC) was carried out, and the results of the immunoassay were in accordance with that of HPLC.
Acknowledgements
This study was supported by Drafting Project of Chinese Agricultural Standard (2007-124) and Fundamental Research Funds for Chinese Central Public-interest Institution (No. 0032011011). The authors expressed sincere gratitude to Chinese Ministry of Agriculture for the financial support. The authors state that there are no conflicts of interest. Contribution made by each author to the research is as follows: M.J. is a PhD researcher who contributed to the design of immunoassay experiment. Z.J. is the professor, specialist in the detection of nutrition component. H.S. and F.J. provided technical assistance for the HPLC analysis. T.C. helped to finish the statistical analysis and revise the paper. J.W. is the professor and is an expert in the study of nutritional application and food safety.