Development of novel biomimetic enzyme-linked immunosorbent assay method based on Au@SiO₂ nanozyme labelling for the detection of sulfadiazine

ABSTRACT

In the current study, a rapid and novel biomimetic nano-enzyme-linked immunosorbent assay has been established for the detection of sulfadiazine (SDZ) using Au@SiO₂ nanoparticles labelling as a marker. In this system, gold nanoparticles exhibited intrinsic peroxidase and activity with excellent water-solubility, and the SiO₂ nanoparticles as ideal carries possessed a large surface area and ease of surface functionalization. The molecularly imprinted polymer films were selected as bionic antibody because of its advantages of selective recognition and reusability. Combine the above advantages, the method exhibited good stability, recognition capability and sensitivity. Under the optimal condition, the limit of detection of the immunosorbent assay was 0.2 mg/L. Beef sample spiked with SDZ was analysed, and the recoveries were in the 78.00% to 90.96% range. The assay could be used to detect SDZ residues in pork and chicken samples, and the results were not significantly different from those obtained by high-performance liquid chromatography.

KEYWORDS:

• Nanozyme
• Au@SiO2 nanoparticles
• molecularly imprinted polymer film
• biomimetic immunosorbent assay
• sulfadiazine

Disclosure statement

No potential conflict of interest was reported by the author(s).

Ethical approval

This article does not contain any studies with animals performed by any of the authors.

Informed consent

Informed consent was obtained from all individual participants included in the study.

Additional information

Funding

Dr. Zhixiang Xu has received research grants from the National Natural Science Foundation of China (grant number 31972149) and Key R & D Project of Jining (grant number 2019NYNS002).