Gynostemma pentaphyllum polysaccharide prevents the growth of h22 ascites tumour by enhancing immunity rather than cytotoxicity in mice

ABSTRACT

Polysaccharide from Gynostemma pentaphyllum Makino (GPP) could prevent H22 tumour growth and have immunostimulatory in mice, but the mechanism is largely unknown. In this study, we further investigated whether GPP prevents tumour growth by inducing cytotoxicity to the tumour cells or enhancing immunity in H22 ascites tumour-bearing mice. The results showed that GPP prevented the H22 ascites tumour growth in vivo, but showed little effect on cell viability and cycles to H22 cells in vitro. GPP improved the proportion and mitochondrial level of T cells, and promoted the secretion of IL-2 and IFN-γ in the ascites of the mice. Furthermore, GPP in vitro enhanced the cell viability and promoted activation (in particular T cells), proliferation, and the secretion of IL-2 and IFN-γ of lymphocyte. Taken together, our results demonstrated that GPP prevents H22 ascites tumour growth by enhancing immunity rather than cytotoxicity in mice.

KEYWORDS:

• Gynostemma pentaphyllum polysaccharide
• enhancing immunity
• h22
• T cells
• cytotoxicity

Disclosure statement

No potential conflict of interest was reported by the author(s).

Additional information

Funding

This work was supported by Characteristic Innovation Projects of Colleges and Universities in Guangdong Province – Innovation and Strengthening School Project from Guangdong Pharmaceutical University [grant number 2018KTSCX104], Innovation Team Project of Universities in Guangdong Province [grant number 2018GKCXTD005], Medical Science and Technology Foundation of Guangdong Province [grant number A2019549], and Natural Science Foundation of Guangdong Province [grant number 2019A1515011818].