Development and validation of an immunochromatography test strip for rapid detection of pyrimethanil residues

ABSTRACT

Pyrimethanil is one of anilinopyrimidine fungicides widely used in crop protection and frequently found in a variety of crops, including fruits, vegetables, and cereals. This paper describes the development of the first reported immunochromatography test strip (ICTS) based on colloidal gold-labeled monoclonal antibody for the detection of pyrimethanil. Under optimal conditions, the visual cut-off value of ICTS for pyrimethanil was 40 ng/mL. Using a portable strip reader, the 50% inhibition concentration and calculated LOD of pyrimethanil in the PBS samples were 4.8 and 0.9 ng/mL, respectively. When used to analyse fortified samples of fruit and vegetable, acceptable recovery rates of 83.3–105.7% with the coefficient of variation values below 11.7% were obtained. Analysis of blindly fortified samples by the proposed ICTS gave similar results to those of the ic-ELISA and HPLC-MS/MS methods. These results indicate the proposed ICTS is a rapid, simple analytical method for quantitative on-site monitoring of pyrimethanil in foodstuffs.

KEYWORDS:

• Pyrimethanil
• immunochromatography test strip
• colloidal gold
• fungicide residues
• food analysis

Disclosure statement

No potential conflict of interest was reported by the authors.

Additional information

Funding

The authors would like to think the National Natural Science Foundation of China [grant number 31671939]; the Science and Technology Research Program of Chongqing Municipal Education Commission [KJZD-K201800501]; the Scientific and Technological Research Project of Chongqing [cstc2018jscx-msybX0201] and the Postgraduate Scientific Research and Innovation Project of Chongqing Municipal Education Commission [CYS19297].