Coating and storing enzyme‐linked immunosorbent assay plates used for analysis of avian pathogens to give a long shelf‐life

Abstract

Enzyme‐linked immunosorbent assays (ELISAs) were developed for the detection of antibodies to Mycoplasma synoviae, Pasteurella multocida, reovirus, infectious bursal disease virus (IBDV), infectious bronchitis virus (IBV), and egg‐drop syndrome (EDS). Purified antigenic preparations derived from the various pathogens were used to coat microtitre plates and their shelf‐life was determined. There was no significant difference in ELISA absorbance values determined on paired antigen coated microtitre plates stored at —20°C and at room temperature, desiccated, for 63 days. When stored at 4°C, desiccated, antigen coated plates had a shelf‐life of up to 390 days. A linear relationship was established between the two sets of values with correlation coefficients ranging from 0.796 to 0.993.

Keywords:

• ELISA
• viral antigens
• bacterial antigens
• poultry