Application of an automated particle‐based immunosensor for the detection of aflatoxin B₁ in foods

Abstract

A method using an automated particle immunosensor is described which can easily detect aflatoxin B₁ down to a level of 4 ng g^‐1 (4 ppb) in reference food materials. The assay takes approximately 8 min and employs a simple extraction procedure which takes <60 min to complete. Experiments with other aflatoxins indicate that only G₂ shows significant cross‐reactivity (23%). The potential of the method for both surveillance and application in the food industry is assessed.

Keywords:

• Aflatoxin B₁
• food
• particle immunoassay
• mycotoxins
• immunosensor