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Abstract
An ELISA method to screen the fungicide metalaxyl in wine was developed. To help prevent matrix effects, wine samples were diluted 1: 10 in a solution of sodium bisulfite, dibasic sodium phosphate and polyvinylpyrrolidone. To determine the accuracy and reproducibility of the method, spiked wine samples were employed. Percentage recoveries ranged from 85.3 to 112, while intra‐assay percentage coefficients of variation (%CVs) varied from 6.5 to 16 and inter‐assay %CVs ranged from 10 to 22. A comparison between highperformance liquid chromatography and ELISA was examined utilizing 32 samples. A correlation coefficient of 0.9919 was obtained.
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