Immunomethods for detecting a broad range of polychlorinated biphenyls

Stephen A. Shelton Department of Molecular and Cell Biology, Institute of Medical Sciences, University of Aberdeen, Foresterhill, Aberdeen, AB25 2ZD, UK

Barbara M. Graham Department of Molecular and Cell Biology, Institute of Medical Sciences, University of Aberdeen, Foresterhill, Aberdeen, AB25 2ZD, UK

Gillian Strachan Department of Molecular and Cell Biology, Institute of Medical Sciences, University of Aberdeen, Foresterhill, Aberdeen, AB25 2ZD, UK

Helen Dooley Department of Molecular and Cell Biology, Institute of Medical Sciences, University of Aberdeen, Foresterhill, Aberdeen, AB25 2ZD, UK

Andrew J. Porter Department of Molecular and Cell Biology, Institute of Medical Sciences, University of Aberdeen, Foresterhill, Aberdeen, AB25 2ZD, UK

William J. Harris Department of Molecular and Cell Biology, Institute of Medical Sciences, University of Aberdeen, Foresterhill, Aberdeen, AB25 2ZD, UK; Phone: 01224 273185

Abstract

Aroclor 1254 (consisting of a mixture of over 100 different congeners of polychlorinated biphenyls (PCBs) was adsorbed on to the surface of polystyrene microbeads and used to immunize mice and rabbits. High titre antisera were produced. PCBs were also adsorbed on to antimony pentoxide microbeads which were then used effectively as immobilized antigen in microbead‐based ELISA assays. Antisera raised with Aroclor 1254‐coated beads contained antibodies with high affinity (as determined by BIAcore analysis) against tetracholoro‐biphenyls even though these congeners represent only a minor fraction in Aroclors.

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Immunomethods for detecting a broad range of polychlorinated biphenyls

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Immunomethods for detecting a broad range of polychlorinated biphenyls

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