A mathematical model to predict the effects of temperature on timed ELISA

Abstract

Timed ELISA is a protocol that enables quantitative ELISA without the aid of sophisticated laboratory equipment. It is likely be used outside of a temperature‐controlled environment and the response of this system to temperature is therefore significant. A model of Timed ELISA, based on the expected performance of its individual reactions, was able to predict the performance of the whole system between 15 and 25°C. Both the model and experimental results confirmed that Timed ELISA possessed an element of temperature compensation, although antigen standards were still required for accuracy. Interrogating the model with regards to a more thermostable conjugate and increased specific activity predicted that the latter would be most suited to extending the temperature range of this system.

Keywords:

• ELISA
• model
• temperature
• Timed ELISA