Comparative study of radioprotective effects of selenium nanoparticles and sodium selenite in irradiation-induced nephropathy of mice model

Abstract

Purpose: The current study was undertaken to evaluate radioprotective effects of selenium (Se) nanoparticles in irradiation-induced nephropathy of mice model compared to sodium selenite.

Materials and methods: Forty-five mice were divided into three major groups including control, Se nanoparticle, and sodium selenite. Each major group was further subdivided into three more groups receiving various doses of 0, 2, and 8 Gy gamma irradiation. Both of the supplements were administered intraperitoneally with the dose of 0.1 mg/kg for 14 consecutive days. At the end of each week, the animals were exposed to gamma radiation and 48 h after the last exposure, the animals were humanely euthanized, then blood and renal tissue samples were taken. Serum creatinine, urea, cystatin C, and beta-2-microglobulin levels as well as activities of renal antioxidant enzymes, superoxide dismutase, glutathione peroxidase and catalase, also malondialdehyde level, total antioxidant capacity, renal tissue Se content, and histopathological features were assessed.

Results: The results showed that both of the supplements could normalize aforementioned indices. However, selenium nanoparticles (Se-NPs) were more effective than sodium selenite.

Conclusions: Conclusively, Se-NPs as an emerging potent antioxidant agent can protect against irradiation-induced nephropathy.

Keywords:

• Irradiation
• selenium
• kidney
• nanoparticle
• oxidative stress
• mice

Acknowledgements

This study would not have been possible without enthusiastic participation and support of Mr. H. Jafari-Namin and Dr. Bahram Golmordi. We sincerely thank them.

Disclosure statement

No potential conflict of interest was reported by the authors.

Notes on contributors

Masoumeh Karami, MS, PhD, is an assistant professor of Clinical Biochemistry, AJA University of Medical Sciences.

Siamak Asri-Rezaei, DVM, PhD, is an associate professor of Clinical Pathology and dean of department of Clinical Pathology and Internal Medicine, Faculty of Veterinary Medicine, Urmia University.

Banafshe Dormanesh, MD, is an associate professor of Pediatric Nephrology and director of research deputy of AJA University of Medical Sciences.

Ali Nazarizadeh, DVM, is a graduate student of Urmia University and holder of young talent prize form Iranian National Elites Foundation.

Additional information

Funding

This study is a part of military service research project of Dr. Ali Nazarizadeh under supervision of Drs. Karami, Asri, and Dormanesh. Ali Nazarizadeh would like to highly appreciate the financial support received from AJA University of Medical Sciences.