http://orcid.org/0000-0002-0002-8471
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Abstract
Purpose: The aim of this study is to investigate the effects of low frequency and intensity sinusoidal magnetic field (SMF) and pulsed magnetic field (PMF) exposure on the chronological aging and cellular stability of Saccharomyces cerevisiae.
Materials and methods: The S. cerevisiae wild type strain (WS8105-1C) was exposed to SMF (2.45 mT, 50 Hz, continuous) and PMF (1.5 mT, 25 Hz, 8 h/day). Chronological aging was evaluated during 40 days. Survival was assayed by clonogenic assay and drop test. Cellular stability was studied by spontaneous mutation count and the index of respiratory competence (IRC).
Results: We found that exposure to PMF produces an acceleration of cellular chronological aging, not observed in the groups treated with SMF. A decrease in the spontaneous frequency of mitochondrial mutation during aging was observed in PMF-treated samples. However, no alterations in the IRC during aging were found for both, SMF and PMF, treatments.
Conclusions: Exposure to PMF produces the acceleration of aging and an alteration in cellular stability.
Acknowledgements
The authors express their gratitude to Dr A.A. Friedl (Department of Radiation Oncology, Ludwig-Maximilians-Universität München, Munich, Germany) for kindly providing the yeast strain. The authors also thank Ms. L. Gil Carmona (Universidad de Málaga, Spain) for her technical assistance.
Disclosure statement
No potential conflict of interest was reported by the authors.
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Notes on contributors
Silvia Mercado-Sáenz
Silvia Mercado-Sáenz, MSc PhD, is an Instructor of Histology at the Department of Human Physiology, Human Histology, Pathology and Sports Physical Education, Faculty of Medicine, University of Málaga, Málaga, Spain. Her research interests include biological and health effects of non-ionizing radiation using experimental approaches. Other interests focus on cellular aging, DNA sequencing, genetic analysis and metagenomics.
Antonio M. Burgos-Molina
Antonio M. Burgos-Molina, MEd MSc PhD, is an Instructor of Immunology and Immunopathology at the Department of Surgical Specialties, Biochemistry and Immunology, Faculty of Medicine, University of Málaga, Málaga, Spain. His research interests include biological and health effects of non-ionizing radiation using experimental approaches. Other interests focus on Immunology.
Beatriz López-Díaz
Beatriz López-Díaz, MSc PhD, currently is a member of the research group at the Department of Radiology and Physical Medicine, Faculty of Medicine, University of Málaga, Málaga, Spain. Her research interests focus on experimental investigation of biological and health effects of electromagnetic fields.
Francisco Sendra-Portero
Francisco Sendra-Portero, MD PhD, is an Associate Professor of Radiology and Physical Medicine and Department Chair, at the Department of Radiology and Physical Medicine, Faculty of Medicine, University of Málaga, Málaga, Spain. His research interests include biological and health effects of non-ionizing radiation using experimental approaches. Other interests focus on medical imaging, medical education research and e-learning.
Miguel J. Ruiz-Gómez
Miguel J. Ruiz-Gómez, MSc PhD, is an Associate Professor of Radiology and Physical Medicine and Head of the Radiobiology research group, at the Department of Radiology and Physical Medicine, Faculty of Medicine, University of Málaga, Málaga, Spain. His research interests include biological and health effects of non-ionizing radiation using experimental approaches. Other interests focus on molecular radiobiology, cellular aging and biological dosimetry.