Studies on the Structuredness of Cytoplasm and Rates of Enzymatic Hydrolysis in Growing Yeast Cells

Summary

Changes in the structuredness of cytoplasm after irradiation with uv-light under oxic and hypoxic conditions in yeast cells were studied. These were followed by measuring the fluorescence polarization of fluorescein molecules produced by enzymatic hydrolysis of the fluoresceindiacetate (FDA) in the cytoplasm of cells in logarithmic growth. Concomitant changes in the rates of enzymatic hydrolysis of FDA to fluorescein in vivo were measured. Mechanisms for the photolytic changes in the structuredness of the cytoplasm and in the rates of enzymatic hydrolysis of FDA are discussed.