Implication of Tryptophan and Tyrosine in the Binding of P-nitrophenyl-α-D-mannopyranoside by Concanavalin A. A Radiolytic Study

Summary

Equilibrium dialysis has been used to study the effects of ⁶⁰Co γ-radiolysis on the carbohydrate binding sites of Con A. Reaction of e⁻_aq and OH· with Con A is accompanied by a decrease in the number of binding sites. Modification of the binding site by (Br) is independent of the initial concentration of Br⁻. Reaction of (SCN) with the protein, however, is dependent on the initial SCN⁻ concentration. The results imply that tryptophan and possibly tyrosine are probably involved in the carbohydrate binding process.