E.S.R. of Spin-trapped Radicals in γ-irradiated Polycrystalline Amino Acids, N-acetyl Amino Acids and Dipeptides

Summary

The radicals produced in several polycrystalline amino acids, N-acetyl amino acids and dipeptides by γ-radiolysis at room temperature were investigated by spin-trapping. After irradiation in the solid state, the samples were dissolved in aqueous solutions of t-nitrosobutane and the trapped radicals identified by e.s.r. For α-amino acids, deamination radicals were found, and in some cases H-abstraction radicals were also observed. No decarboxylation radicals could be detected. For N-acetyl amino acids, except for N-acetylglycine, the major radical was the decarboxylation radical. For N-acetyglycine the H-abstraction radical from the glycine residue was observed. For dipeptides of the type x-glycine, the radical formed by removal of H from the α-carbon of the carboxyl-terminal residue was always spin-trapped. Some primary deamination radicals and minor amounts of decarboxylation radicals could also be observed. For dipeptides of the type x-alanine, glycine-x and alanine-x, the decarboxylation radical was always the major spin-trapped radical. Some primary and secondary deamination radicals were also detected.