Sensitization of Escherichia Coli B/r to X-irradiation by 2′-chloro-2′-deoxythymidine

Summary

The sensitizing effect of 2′Cl-TdR has been investigated from the viewpoint of the formation of free radicals at the C-2′ position of 2′Cl-TdR when reacted with hydrated electrons. E. coli B/r cells were incubated in growth medium containing 2′Cl-TdR. Centrifugation experiments using CsCl equilibrium density gradients were carried out to confirm the incorporation of 2′Cl-TdR into DNA of the cells. The sedimentation profiles of DNA from cells grown in a medium containing 2′Cl-TdR were found at relatively heavier density positions in comparison with those of DNA from control cells. This result confirms that 2′Cl-TdR was incorporated into DNA. E. coli B/r cells which incorporated 2′Cl-TdR were more sensitive than the control cells for killing by X-irradiation under aerobic conditions. On the other hand, no difference in U.V.-inactivation curves between the cells grown in growth medium containing 2′Cl-TdR and the control cells was observed.

From experiments using alkaline sucrose density gradient centrifugation, an increase in the frequency of radiation-induced single strand breaks of E. coli DNA containing 2′Cl-TdR was observed, compared with those of DNA from control cells. These results reveal that the sensitization of 2′Cl-TdR originates from an increase of damage at the sugar moiety in DNA.

• sensitization
• 2′-chloro-2′-deoxythymidine
• Escherichia coli