13
Views
19
CrossRef citations to date
0
Altmetric
Original Article

Effect of Hyperthermia on the Number of Platinum Atoms Binding to DNA of HeLa Cells Treated with 195mPt-radiolabelled cis-diaminedichloroplatinum(II)

, , , , &
Pages 215-220 | Received 31 Aug 1993, Accepted 28 Apr 1994, Published online: 03 Jul 2009
 

Abstract

HeLa S-3 cells were treated with 195mPt-radiolabelled cis-diaminedichloroplatinum(II) (CDDP) for 60 min at various temperatures to examine the relationship between the lethal effect and the number of Pt atoms binding to DNA, RNA and protein molecules. The mean lethal concentration (D0) of CDDP for 60-min treatment at 0, 25, 37, 40, 42 and 44°C was 233, 69·9, 15·9, 11·7, 8·3 and 4·7 µm respectively. By using identically treated cells, the number of Pt atoms combined with DNA, RNA and protein molecules was determined in the subcellular fractions prepared by the method of Schneider (1961). Thus, the D0's given as the drug concentrations were substituted for the number of Pt atoms combined with each fraction. Then the efficiency of the Pt atom to kill the cells was expressed as the reciprocal of the number of Pt atoms combined and was calculated for each molecule. The efficiency for DNA was 2·47, 2·75, 9·49, 9·66, 10·53 and 15·00 × 104 nucleotides respectively for the conditions described above. A detailed comparison of the D0's and efficiencies suggested that the supra-additive effect of the combination treatment could be explained by two mechanisms, i.e. the increased drug level in DNA (from 37 to 42°C) and the increased efficiency of the Pt atoms to kill the cells (> 42°C).

Reprints and Corporate Permissions

Please note: Selecting permissions does not provide access to the full text of the article, please see our help page How do I view content?

To request a reprint or corporate permissions for this article, please click on the relevant link below:

Academic Permissions

Please note: Selecting permissions does not provide access to the full text of the article, please see our help page How do I view content?

Obtain permissions instantly via Rightslink by clicking on the button below:

If you are unable to obtain permissions via Rightslink, please complete and submit this Permissions form. For more information, please visit our Permissions help page.