Increased Frequency of Sister Chromatid Exchanges in Lymphocytes of Chernobyl Clean-up Workers

Abstract

Sister chromatid exchanges (SCEs) were analysed in lymphocytes from 12 control persons and 33 Chernobyl clean-up workers. The group of Chernobyl clean-up workers consisted of civilians who were forced to go to Chernobyl to clean up environmental contamination caused by Chernobyl disaster. On average, they received 0·13 (range 0·04–0·249) Gy of external irradiation before returning to home. Cytogenetic analyses were performed 6–8 years after the irradiation. Standard cytogenetic techniques were used. Mean SCE frequency was 7·45 ± 0·69 SCE/cell in controls and 10·30 ± 0·31 SCE/cell in clean-up workers (p < 0·05). Analysis of variance showed that exposure to radiation explained 19·6%, occupational exposure to various chemical substances, 11·9%, coffee consumption, 8·3%, smoking, 4·2%, interaction between smoking and coffee consumption, 3·6%, and alcohol abuse, 3·4% of total variation in SCE frequency. Effects of all above confounding factors were significant (p < 0·05). In addition, increased frequencies of chromosome aberrations due to exposure at Chernobyl and alcohol consumption were observed. However, there was no correlation between external dose of irradiation and the frequency of chromosome aberrations. Thus, even 6–8 years after the irradiation, cytogenetic effects in lymphocytes of Chernobyl clean-up workers are still significant.