Voltametric measurement of blood nitric oxide in irradiated rats

Abstract

Purpose: To investigate the effect of blood nitric oxide (NO) as a mediator of the neurovascular syndrome in rats following gamma -irradiation. Material and methods: Using a voltametric method together with a carbon fibre based sensor, NO measurements were carried out in sham-irradiated and irradiated animals either in blood from the abdominal aorta or in blood samples from the heart. Results: In in vitro conditions, properties of the probe were not altered by the ionizing radiation. Significant increases of 17% and 25.6% were observed in the voltametric signal height at 90min and 24h respectively after a 15Gy gamma-ray exposure. These effects were followed on days 3 and 4 by a progressive decrease in the signal height of 7% and 18% respectively. Dose-effect relationships were observed at 90min and 24h after exposure to gamma-rays in the range of 3-15Gy. Finally, the NO dependence on the measured voltametric signal was controlled by using inhibitors of the NO synthase (NOS) and by performing nitrate assays. Conclusions: Specific blood NO voltametric measurements are possible. Functional changes associated with NO after gamma-ray exposure are discussed.