Detection of procymidone in wine and grapes by a magnetic particle‐based enzyme immunoassay

Abstract

A competitive enzyme immunoassay has been developed to detect procymidone in wine at the parts per billion level (ppb) without sample dilution or extraction. This immunoassay utilizes magnetic particles as the solid phase which allows for the precise addition of antibody and rapid reaction kinetics. The sensitivity of the assay based on 90% B/Bo is 0.8 ppb in wine. The recovery of 30 wine samples spiked with four levels of procymidone averaged 104%. The specificity of the polyclonal antibody used allows for the quantitation of procymidone in the presence of other commonly applied fungicides including vinclozolin, iprodione, captan and carbendazim. Correlation of 27 wine samples by the immunoassay method and a GC/ ECD procedure yielded a regression (r) of 0.972. Recovery studies indicate that this immunoassay can also be used to detect procymidone on grapes. The average recovery of procymidone from grapes was 113%.