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Articles

Decrease of inhibitory effect of 2-chlorophenol on nitrification in sequencing batch reactors

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Pages 3422-3433 | Received 11 Jan 2018, Accepted 05 May 2018, Published online: 24 May 2018
 

ABSTRACT

The metabolic and kinetic behaviour of a nitrification process in the presence of 2-chlorophenol (2-CP) was evaluated in two sequencing batch reactors (SBR1, SBR2) inoculated with nitrifying sludge previously exposed to phenolic compounds. The SBR1 was inoculated with sludge previously exposed to 2-CP, while the SBR2 was inoculated with sludge previously exposed to p-cresol. An inhibitory effect of 20 mg 2-CP-C/L on both nitrification processes was observed, as specific rates decreased according to a control assay in the absence of 2-CP. However, the inhibitory effect decreased throughout the cycles. At the end of cycle 6, a stable nitrifying process was observed with the sludge previously exposed to 2-CP (SBR1), as an ammonium consumption efficiency and a nitrate production yield close to 99.6 ± 0.3% and 0.99 ± 0.02 were respectively achieved. Despite a complete ammonium consumption being achieved with the sludge previously exposed to p-cresol (SBR2), partial nitrification was observed as nitrate production yield accounted for 0.28 ± 0.08 and nitrite was accumulated within the culture. Nevertheless, both nitrifying sludges had the ability to completely consume 2-CP. The use of SBR systems with nitrifying sludge previously exposed to 2-CP resulted in a better nitrification performance, thus it may be a good alternative for achieving a stable nitrifying respiratory process where complete and simultaneous ammonium and 2-CP consumption can be acquired.

GRAPHICAL ABSTRACT

Disclosure statement

No potential conflict of interest was reported by the authors.

Additional information

Funding

M.A. Martínez-Jardines received an M.D. fellowship from the Council of Science and Technology of Mexico (Consejo Nacional de Ciencia y Tecnología) [grant number CONACYT-CB-2015-01 255740]; Consejo Divisional de Ciencias Biológicas y de la Salud, Universidad Autónoma Metropolitana-Iztapalapa.

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