Differences in carbon source utilization of Salmonella Oranienburg and Saintpaul isolated from river water

Abstract

Long-term exposure to river water by non-indigenous micro-organisms such as Salmonella may affect metabolic adaptation to carbon sources. This study was conducted to determine differences in carbon source utilization of Salmonella Oranienburg and Salmonella Saintpaul (isolated from tropical river water) as well as the control strain Salmonella Typhimurium exposed to laboratory, river water, and host cells (Hep-2 cell line) growth conditions. Results showed that Salmonella Oranienburg and Salmonella Saintpaul showed better ability for carbon source utilization under the three growth conditions evaluated; however, S. Oranienburg showed the fastest and highest utilization on different carbon sources, including D-Glucosaminic acid, N-acetyl-D-Glucosamine, Glucose-1-phosphate, and D-Galactonic acid, while Salmonella Saintpaul and S. Typhimurium showed a limited utilization of carbon sources. In conclusion, this study suggests that environmental Salmonella strains show better survival and preconditioning abilities to external environments than the control strain based on their plasticity on diverse carbon sources use.

Keywords:

• Salmonella
• metabolism
• environment
• stress response

Acknowledgments

Authors thank to Consejo Nacional de Ciencia y Tecnología (CONACyT) and Convocatoria de Investigación Científica Básica SEP-CONACyT 2010 for its financial support. The authors also thank to Food Safety Research National Laboratory at the Centro de Investigación en Alimentación y Desarrollo, Unidad Culiacán, and Bs. Célida Martínez for the technical support during this research.