Studies on the toxic effects of agrochemical pesticide (Monocrotophos) on physiological and reproductive behavior of indigenous and exotic earthworm species

ABSTRACT

Earthworms are an ideal biological model in toxicity assays and environmental monitoring studies. In the present study, the reproductive toxicity and histopathological effects of Monocrotophos pesticide on an exotic epigeic Eudrilus eugeniae and an indigenous epigeic Perionyx barotensis earthworm were studied. Earthworm species were exposed to different concentrations of pesticide like 450 ppm, 500 ppm, and 650 ppm for 45 days and the mortality rate and reproductive activity was recorded every 15 days of exposure. There was an increase in mortality and abnormal sperm (asthenospermia, necrospermia, and oligospermia) and defective cocoons in earthworms with increasing concentrations of the pesticide. Histopathological changes like rupture of chloragogenous tissue, longitudinal muscle, fused and extra-villous growth and necrotic cell rupture in earthworm’s body wall (epidermis, circular and longitudinal muscles) were observed. Fluorescent probes have detected cell death in pesticide-treated earthworms when compared to the control group after 45 days. The present findings show that Monocrotophos pesticide on exposure to epigeic earthworm species causes significant reproductive toxicity and histopathological abnormalities and these changes could be used as a tool in environmental risk assessment of pesticides.

Abbreviations: DDT: Dichlorodiphenyltrichloroethane; MCP: Monocrotophos; EPA: Environment Protection Act; SL: Soluble Liquid; C: N (Carbon: Nitrogen); C: P (Carbon: Phosphorus); LC: Lethal Concentration; PBS: Phosphate Buffer Solution; WHO: World Health Organization; H&E: Hematoxylin and Eosin; SV: seminal vesicles; O: ovary; GP: genital papillae; Ch: chloragogenous tissue; EL: epithelial layer; CM: circular muscle; LM: longitudinal muscle; CD: cell debris

Graphical Abstract

Toxic Effects of Agrochemical Pesticide (Monocrotophos) on Physiological and Reproductive Behavioral changes in Indigenous and Exotic Earthworm Species

KEYWORDS:

• Eudrilus eugeniae
• Perionyx barotensis
• Monocrotophos
• Reproductive toxicity
• Histopathology

Acknowledgments

The authors wish to thank the authorities of Periyar University for providing the necessary facilities to carry out the research work.

Disclosure statement

No potential conflict of interest was reported by the authors.

Correction Statement

This article has been republished with minor changes. These changes do not impact the academic content of the article.