Synthesis of silver and copper nanoparticle using Spirulina platensis and evaluation of their anticancer activity

ABSTRACT

The present research displays the green synthesis of stable silver nanoparticles (Ag-NPs) and copper oxide nanoparticles (CuO-NPs). The aqueous solution of Spirulina platensis (blue green algae) source was used as a reducing and capping agent and this study assessed the cytotoxicity of Ag- and CuO-NPs on three cancer cell cultures: A549 (lung cancer), HCT (human colon cancer), Hep2 (laryngeal carcinoma cancer) and normal cell (WISH). For NPs characterization, the UV/Vis spectroscopy was used where their formation and crystallinity were proven with $\mathit{\lambda }$max values for Ag- and CuO-NPs of 425 and 234 nm, respectively. According to X-ray diffraction and transmission electron microscopy (TEM), Ag-NPs were spherical in shape (size 2.23–14.68 nm) and CuO-NPs were small (size 3.75–12.4 nm). Zeta potential analysis showed the particles potential, which was recorded by −14.95 ± 4.31 mV for Ag-NPs and −21.63 ± 4.90 mV for CuO-NPs. After that, Ag- and CuO-NPs were assessed for anticancer properties against A549, HCT, Hep2 and WISH. IC₅₀ of Ag-NPs recorded 15.67, 12.94, 3.8 and 10.44 µg/ml for WISH, A549, HCT and Hep2, respectively. IC₅₀ for CuO-NPs was recorded as 32.64, 54.59, 3.98 and 20.56 µg/ml for WISH, A549, HCT and Hep2 cells, respectively. Safety limits for WISH and A549 were achieved 98.64% by 2.44 µg/ml and 83.43% by 4.88 µg/ml of Ag-NPs, and it was found to be 97.94% by 2.44 µg/ml against HCT, while that for Hep2 is 95.9% by 2.44 µg/ml. Concerning the anticancer effect of CuO-NPs, the safety limit was recorded as 88.70% by 2.44 and 98.48% by 4.88 µg/ml against WISH and A549, while HCT reached 89.92% by 2.44 µg/ml and Hep2 was 83.33% by 4.88 µg/ml. Green nanotechnology applications such as Ag-NPs and CuO-NPs have numerous benefits of ecofriendliness and compatibility for biomedical applications such as anticancer effects against cancer cells.

KEYWORDS:

• Nanoparticles
• Spirulina platensis
• Ag-NPs
• CuO-NPs, anticancer

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No potential conflict of interest was reported by the authors.

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Funding

The author(s) reported there is no funding associated with the work featured in this article.