https://orcid.org/0000-0003-1523-7759
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ABSTRACT
Tetraclinis articulata essential oil proved to be effective in controlling tomato grey mould, so we would investigate its effect on some tomato defense mechanisms. The pretreatment of Botrytis cinerea infected tomato plants with TAEO emulsion enhanced the activity of antioxidant enzymes activity SOD, CAT, APX, and GPX, and total polyphenols content and it decreased IC50 of free radical-scavenging activity and H2O2 content. Results showed amelioration in antioxidant status in TAEO emulsion treated and B. cinerea infected plants indicating that treatment decreased infection in tomato plants. The qRT-PCR analysis of defense genes expression Chitinase SlChi, transcription factors SlWRKY and SlAP2/ERF, Lipoxygenase SlLOX, and Thioredoxin SlTRX showed that they were up-regulated as early as 12 hpi sustained with a second increase at 48 hpi in TAEO emulsion pretreated and infected plants. These results suggest the potential use of TAEO emulsion as natural product to induce tomato antioxidant status and activate defense genes.
Disclosure statement
No potential conflict of interest was reported by the author(s).
Abbreviation list
| APX | = | Ascorbate peroxydase |
| B. cinerea | = | Botrytis cinerea |
| CAT | = | Catalase |
| CHI | = | Chitinase |
| EO | = | Essential oil |
| ERF | = | Ethylene response factor |
| FG | = | Fongicide |
| FM | = | Fresh matter |
| GAE | = | Equivalent acide gallique |
| GC-MS | = | Gas chromatography coupled with mass spectrometry |
| GPOX | = | Guaïacol peroxydase |
| HPI | = | Hour after inoculation |
| IC50 | = | Inhibition concentration at 50% |
| LOX | = | Lipoxygénases |
| MW | = | Molecular wight |
| PCR | = | Polymerase chain reaction |
| ROS | = | Reactive oxygenated species |
| RP-HPLC-DAD-ESI-MSn | = | Chromatographie liquide en phase inverse couplée à la spectrométrie de masse par électro-nébulisation |
| SOD | = | Superoxyde dismutase |
| T. articulata | = | Tetraclinis articulata |
| TAEO | = | Tetraclinis articulata essential oil |
| TRX | = | Thioredoxine |