ABSTRACT
The cockroach is one of the most important disease vectors in world. Entomopathogenic fungi, as three concentrations of spores were taken 1.1 × 105, 1.1 × 107, and 1.1 × 109 conidia/mL from two isolates of Nour and Saravan-Iranian. In this study, the immersion method caused about 13% mortality only in isolation (1 × 109 conidia/mL) of Saravan isolates. Inoculation of isolates below the pronotum did not significantly differ the mortality rate between the two genera (P = 0.8), compared to the pathogenicity of three isolates of M. anisopliae (1.1 × 105, 1.1 × 107, and 1.1 × 109 conidia/mL). In total, Saravan and Nour isolates were 66%, 73%, and 93%, respectively, indicating a significant difference (P < 0.001). Mortality of male and female cockroaches with Saravan isolates respectively occurred 3 and 4 days after inoculation (LT50 = 4.3d), while for Nour isolates, in both sexes, mortality was observed within four days after the test (LT50 = 5.5d). Considering the results M. anisopliae can be one benefit methods for control American cockroach in the future.
Acknowledgements
Thanks to the research and technology department colleagues of Gonabad University of Medical Sciences who approved the plan.
Disclosure statement
No potential conflict of interest was reported by the author(s).
Author contributions
HR: Conceptualization, Methodology, Formal Analysis, Writing – Original Draft. MGh: Supervision, Conceptualization, writing – Review and Editing, and prepare fungi for the test, HR: Study for References.
OFAC statement
Greetings and respect to you, dear colleagues. We are very happy that we have sent our article to your excellent scientific journal. We, the authors of this article, declare that we do not have any government political affiliation, we are all academic professors, and we have not received any government funding in the implementation of this scientific project. Our goal for this research was to solve an important health problem in Iran and the world. We seek to solve the problems of the people of the world in the shade of peace and tranquility.