Susceptibility of gut indigenous lactic acid bacteria in BALB/c mice to oral administered Lactobacillus plantarum

Abstract

Cells of Lactobacillus plantarum strains AN1 and Tennozu-SU2 exert anti-inflammatory responses in ICR mouse models of inflammatory bowel disease and protective effects against S. Typhimurium infection in BALB/c mice, respectively. To clarify the existence of L. plantarum-susceptible gut indigenous bacteria, AN1 and Tennozu-SU2 cells were administered to BALB/c mice via drinking water. Gene amplicon sequencing of 16S rRNA of caecal content revealed that the AN1 and Tennozu-SU2 cells affected the abundance of caecal indigenous lactobacilli, but the effect on the dominant Clostridiales and Bacteroidales was not clear. With Blood and Liver (BL) agar containing 5% v/v horse blood, six typical colonies from faecal samples were detected as the principal lactobacilli. Among them, two typical colonies were isolated and identified to be AN1 and Tennozu-SU2. Two and one typical colonies detected in all mice were identified to be L. reuteri and L. murinus, respectively. The other one was identified and estimated to be indigenous L. plantarum detected in the Tennozu-SU2 group.

Keywords:

• Lactobacillus plantarum
• gut microbiota
• indigenous lactic acid bacteria
• BALB/c mouse

View correction statement:

Corrigendum

Disclosure statement

The authors declare that they have no conflict of interest.

Additional information

Funding

This work was partially supported by the Agriculture, Forestry, and Fisheries Research Council; Ministry of Agriculture, Forestry, and Fisheries, Japan; Nutrition Act Co., Tokyo, Japan; and the Japan Food Chemical Research Foundation.