Absorption of 1,3-dioleyl-2-palmitoylglycerol and intestinal flora profiles changes in mice

Abstract

The purpose of this study was to investigate the digestion and absorption of 1,3-dioleyl-2-palmitoylglycerol (OPO) and its effects on mineral absorption and intestinal flora in vivo. Fatty acids and mineral faeces were measured using gas chromatography-mass spectrometry and inductively coupled plasma mass spectrometry, respectively. The intestinal flora composition was assessed using high-throughput 16S rRNA sequencing. After 4-week feeding experiment, the apparent absorption rate of calcium in O group (mice fed with OPO) was 48.54 ± 0.21%, which was significantly higher than that in Z group (mice fed with mixed vegetable oil), the percentage of palmitic acid in serum of the O group mice was significantly higher. Compared with Z group, the mean relative abundance of Bifidobacteriaceae and Lactobacillaceae in the O group mice was higher. These results show that OPO can promote the absorption of fat and calcium, promote the growth of beneficial bacteria.

Keywords:

• 1,3-dioleyl-2-palmitoylglycerol
• fatty acid
• mineral
• Sn-2 palmitic acid
• intestinal flora

Acknowledgements

The authors thank Hubei UBT Biological Engineering Co. Ltd for their support and Editage (www.editage.cn) for English language editing.

Disclosure statement

No potential conflict of interest was reported by the author(s).

Additional information

Funding

This work was supported by the National Natural Science Foundation of China [grant number 31671874]; National Key Research and Development Project [grant number 2018YFD0502404]; Natural Science Foundation of Heilongjiang Province of China [grant number C2018022]; and innovative talents special fund from Harbin Science and Technology Bureau [grant number 2016RAXXJ021].