Abstract
Large numbers of protoplasts (106 to 3·2 × 107 per gram fresh weight) were routinely isolated from two Taiwanese species of Porphyra: P. dentata and P. crispata. Intermediate and final forms of regeneration were observed, including swollen cells, monospore-like cells, filaments, callus-like structures and bladelets. Regeneration of leafy plants was achieved from protoplasts of P. crispata, whereas only conchocelis-like filaments were regenerated from protoplasts of P. dentata. In this latter species, conchosporangia and monosporangia appeared on the filaments upon cultivation at 25 °C. The variability in regenerative patterns is discussed.
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