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Abstract
Glutathione S-transferases (GSTs) are important detoxification enzymes in insects. This study examined the inhibitory effects of five insecticides (beta-cypermethrin, fenpropathrin, phoxim, abamectin, acetamiprid) and two plant-derived allelochemicals (quercetin, tannic acid) on GST activity in Micromelalopha troglodyta (Graeser) and Clostera anachoreta (Fabricius). GST activities were suppressed by all the insecticides and allelochemicals tested; the two allelochemicals were the most potent inhibitors, reducing GST activity in vitro in a dosage-dependent manner. Kinetic analyses of GST inhibition by quercetin and tannic acid in M. troglodyta and C. anachoreta suggested biochemical differences in their GSTs.
Acknowledgements
This research was supported by the National Natural Science Foundation of China [Contract No. 30600476, 30972376 and 31370652], a General Financial Grant from the China Postdoctoral Science Foundation [2013M530262] and the Priority Academic Program Development Fund of Jiangsu Higher Education Institutions. We thank Dr. Joe Zhou of Kentucky University and Dr. Steve Chambers, Metro Editor of The Star-Ledger “Daily News” for a critical reading of the English manuscript, and Youhui Gong in China Agricultural University and Xianghua Xu in Nanjing University of Information Science and Technology for the statistical analysis.
