Biochemical and molecular mechanisms of diafenthiuron resistance in the whitefly, Bemisia tabaci

ABSTRACT

B-biotype Bemisia tabaci has developed high levels of resistance to many insecticides. To investigate the risks and explore possible mechanisms of resistance to diafenthiuron in B. tabaci, a 32.8-fold diafenthiuron-resistant strain (R-DfWf) was established after selection for 36 generations compared with the susceptible strain (S-Lab). Biochemical assays showed that the activity of cytochrome P450 towards p-NA was significantly higher (4.37-fold higher) in the R-DfWf strain than in the S-Lab strain. Similarly, the carboxylesterase (COE) activity and glutathione S-transferase (GST) activity were also significantly higher (3.12- and 1.83-fold higher, respectively) in the R-DfWf strain than in the S-Lab strain. The expression of five of seven P450 genes was significantly higher (>3-fold) in the R-DfWf strain than in the S-Lab strain. The expression of COE2 was significantly higher (>2.5-fold) in the R-DfWf than in the S-Lab strain. The expression of GST and GST2 was significantly higher (>2.3-fold) in the R-DfWf than in the S-Lab. Thus, cytochrome P450, COE and GST may appear to be responsible for the resistance to diafenthiuron in B. tabaci. It is also valuable for usage of insecticides for resistance management and control of this species.

KEYWORDS:

• Bemisia tabaci
• diafenthiuron
• resistance
• carboxylesterase (COE)
• glutathione S-transferase (GST)
• cytochrome P450

Acknowledgments

I would like personally thank following people who supported me my sincere gratitude, all authors, reviewers, editor, proofread person, and so on.

Disclosure statement

No potential conflict of interest was reported by the authors.

Additional information

Funding

This work was funded by Special Fund for Agro-scientific Research in the Public Interest high-level personnel launch scientific research projects [grant number 208010616003].