Next-generation sequencing-based clinical metagenomics identifies Prevotella pleuritidis in a diabetic adolescent with large parapneumonic effusion and negative growth of pleural fluid culture: a case report

ABSTRACT

We report a 12-year-old diabetic boy with a right-sided parapneumonic effusion and pneumonia who failed initial empirical antibiotics. Prevotella pleuritidis was identified from the pleural fluid using next-generation sequencing-based clinical metagenomics with cultures of pleural fluid and blood resulting negative. The patient responded well to intravenous meropenem followed by oral metronidazole.

KEYWORDS:

• Parapneumonic effusion
• Prevotella pleuritidis
• next-generation sequencing-based clinical metagenomics

Acknowledgements

We would like to thank Dr Benjamin Briggs and Dr Robert Schlaberg for their support on operational details of laboratory testing.

Disclosure statement

No potential conflict of interest was reported by the authors.