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Original

Effects of 2-hydroxyoleic acid on the structural properties of biological and model plasma membranes

, , , , , , & show all
Pages 46-57 | Received 10 Apr 2007, Published online: 09 Jul 2009
 

Abstract

Genetic hypertension is associated with alterations in lipid metabolism, membrane lipid composition and membrane-protein function. 2-Hydroxyoleic acid (2OHOA) is a new antihypertensive molecule that regulates the structure of model membranes and their interaction with certain peripheral signalling proteins in vitro. While the effect of 2OHOA on elevated blood pressure is thought to arise through its influence on signalling proteins, its effects on membrane lipid composition remain to be assessed. 2OHOA administration altered the lipid membrane composition of hypertensive and normotensive rat plasma membranes, and increased the fluidity of reconstituted liver membranes from hypertensive rats. In spontaneously hypertensive rats (SHR), treatment with 2OHOA increased the cholesterol and sphingomyelin content while decreasing that of phosphatidylserine-phosphatidylinositol lipids. In addition, monounsaturated fatty acid levels increased as well as the propensity of reconstituted membranes to form HII-phases. These data suggest that 2OHOA regulates lipid metabolism that is altered in hypertensive animals, and that it affects the structural properties of liver plasma membranes in SHR. These changes in the structural properties of the plasma membrane may modulate the activity of signalling proteins that associate with the cell membrane such as the Gαq/11 protein and hence, signal transduction.

Abbreviations
SHR=

spontaneously hypertensive rats

WKY=

Wistar Kyoto normotensive rats

2OHOA=

2-hydroxyoleic acid

CHO=

cholesterol

SM=

sphingomyelin

PC=

phosphatidylcholine

PE=

phosphatidylethanolamine

PS=

phosphatidylserine

PI=

phosphatidylinositol

PL=

phospholipid

FA=

fatty acid

SFA=

saturated fatty acid

UFA=

unsaturated fatty acids

MUFA=

monounsaturated fatty acids

PUFA=

polyunsaturated fatty acids

DPH=

1,6-diphenyl-1,3,5-hexatriene

BP=

blood pressure

Abbreviations
SHR=

spontaneously hypertensive rats

WKY=

Wistar Kyoto normotensive rats

2OHOA=

2-hydroxyoleic acid

CHO=

cholesterol

SM=

sphingomyelin

PC=

phosphatidylcholine

PE=

phosphatidylethanolamine

PS=

phosphatidylserine

PI=

phosphatidylinositol

PL=

phospholipid

FA=

fatty acid

SFA=

saturated fatty acid

UFA=

unsaturated fatty acids

MUFA=

monounsaturated fatty acids

PUFA=

polyunsaturated fatty acids

DPH=

1,6-diphenyl-1,3,5-hexatriene

BP=

blood pressure

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