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Abstract
Outer membrane proteins are structurally distinct from those that reside in the inner membrane and play important roles in bacterial pathogenicity and human metabolism. X-ray crystallography studies on >40 different outer membrane proteins have revealed that the transmembrane portion of these proteins can be constructed from either β-sheets or less commonly from α-helices. The most common architecture is the β-barrel, which can be formed from either a single anti-parallel sheet, fused at both ends to form a barrel or from multiple peptide chains. Outer membrane proteins exhibit considerable rigidity and stability, making their study through x-ray crystallography particularly tractable. As the number of structures of outer membrane proteins increases a more rational approach to their crystallization can be made. Herein we analyse the crystallization data from 53 outer membrane proteins and compare the results to those obtained for inner membrane proteins. A targeted sparse matrix screen for outer membrane protein crystallization is presented based on the present analysis.
| Abbreviations | ||
| ADA | = | N-(2-Acetamido)iminodiacetic Acid |
| Bicine | = | N,N-Bis(2-hydroxyethyl)glycine |
| Bis Tris | = | Bis(2-hydroxyethyl)amino-tris(hydroxymethyl)methane |
| CHES | = | 2-(N-Cyclohexylamino)ethane sulfonic Acid |
| EPPS | = | 4-(2-hydroxyethyl)piperazine-1-propanesulfonic acid |
| HEPES | = | N-(2-hydroxyethyl)-piperazine-N′-2-ethanesulfonic acid |
| MES | = | 2-(N-morpholino)ethanesulfonic acid |
| MME | = | Monomethylether |
| MPD | = | 2-methyl-2,4-pentanediol |
| MPEG | = | methoxy polyethylene glycol |
| MOPS | = | 3-{N-morpholino] propanesulfonic acid |
| PEG | = | Polyethylene glycol |
| PIPES | = | Piperazine-1,4-bis(2-ethanesulfonic acid |
| TEG | = | triethylene-glycol |
| Tricine | = | N-[Tris(hydroxymethyl)methyl]glycine |
| Tris | = | 2-Amino-2-(hydroxymethyl)propane-1,3-diol |
| Tris HCl | = | 2-Amino-2-(hydroxymethyl)propane-1,3-diol, hydrochloride, cis-Inostitol, cis-1,2,3,5-trans-4,6-cyclohexanehexol |
| Octyl-POE | = | Octyl-Polyoxyethylene |
| Cymal-1 | = | Cyclohexyl-methyl-D-maltoside |
| Cymal-5 | = | 5-Cyclohexyl-1-pentyl-D-maltoside |
| LDAO | = | Lauryldimethylamine-N-oxide |
| OES | = | N-Octyl-2-Hydroxyethylsulfoxide |
| C8E4 | = | Octyl tetraethylene glycol ether |
| C8E5 | = | Octyl pentaethylene glycol ether |
| C10-DAO | = | Decyl dimethyl amino N-oxide |
| C6-DAO | = | Hexyl dimethyl amino N-oxide |
| C10E5 | = | Decyl pentaethylene glycol ether |
| PDB | = | Research Collaboratory for Structural Bioinformatics Protein Data Bank (www.pdb.org) |
| OM | = | outer membrane |
| IM | = | inner membrane |
| MP | = | membrane protein |
| Abbreviations | ||
| ADA | = | N-(2-Acetamido)iminodiacetic Acid |
| Bicine | = | N,N-Bis(2-hydroxyethyl)glycine |
| Bis Tris | = | Bis(2-hydroxyethyl)amino-tris(hydroxymethyl)methane |
| CHES | = | 2-(N-Cyclohexylamino)ethane sulfonic Acid |
| EPPS | = | 4-(2-hydroxyethyl)piperazine-1-propanesulfonic acid |
| HEPES | = | N-(2-hydroxyethyl)-piperazine-N′-2-ethanesulfonic acid |
| MES | = | 2-(N-morpholino)ethanesulfonic acid |
| MME | = | Monomethylether |
| MPD | = | 2-methyl-2,4-pentanediol |
| MPEG | = | methoxy polyethylene glycol |
| MOPS | = | 3-{N-morpholino] propanesulfonic acid |
| PEG | = | Polyethylene glycol |
| PIPES | = | Piperazine-1,4-bis(2-ethanesulfonic acid |
| TEG | = | triethylene-glycol |
| Tricine | = | N-[Tris(hydroxymethyl)methyl]glycine |
| Tris | = | 2-Amino-2-(hydroxymethyl)propane-1,3-diol |
| Tris HCl | = | 2-Amino-2-(hydroxymethyl)propane-1,3-diol, hydrochloride, cis-Inostitol, cis-1,2,3,5-trans-4,6-cyclohexanehexol |
| Octyl-POE | = | Octyl-Polyoxyethylene |
| Cymal-1 | = | Cyclohexyl-methyl-D-maltoside |
| Cymal-5 | = | 5-Cyclohexyl-1-pentyl-D-maltoside |
| LDAO | = | Lauryldimethylamine-N-oxide |
| OES | = | N-Octyl-2-Hydroxyethylsulfoxide |
| C8E4 | = | Octyl tetraethylene glycol ether |
| C8E5 | = | Octyl pentaethylene glycol ether |
| C10-DAO | = | Decyl dimethyl amino N-oxide |
| C6-DAO | = | Hexyl dimethyl amino N-oxide |
| C10E5 | = | Decyl pentaethylene glycol ether |
| PDB | = | Research Collaboratory for Structural Bioinformatics Protein Data Bank (www.pdb.org) |
| OM | = | outer membrane |
| IM | = | inner membrane |
| MP | = | membrane protein |