Detection of Phenolics in Wheat Straw Treated with White Rot Fungus Stropharia rugosoannulata by use of HPLC

Abstract

High performance liquid chromatography with electrochemical detection (HPLC-ED) was used to determine the phenolic composition (free phenolics, alkali labile and alkali resistant lignin) of wheat straw subjected to solid state fermentation with white rot fungus Stropharia rugosoannulata in a reactor designed to obtain different oxygen concentrations and hence different fungal activities. The wheat straw samples fermented in the presence of high oxygen had higher levels of free phenolics and alkali labile phenolics and lower levels of alkali resistant phenolics. HPLC-ED of free phenolics is a rapid means of monitoring the ligninolytic activity of S. rugosoannulata on wheat straw.

Keywords:

• Lignin degradation and analysis
• wheat straw
• high performance liquid chromatography
• electrochemical detection
• Stropharia rugosoannulata
• phenolics