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Abstract
Cherney, D. J. R, Cherney, J. H. and Volenec, J. J. 1993. Inhibition of structural carbohydrate fermentation by cellulase filtrates of alfalfa. J. Appl. Anim. Res., 3: 19–30
Objectives were to investigate the influence of isolated phenolic-carbohydrate complexes from alfalfa (Medicago sativa L.) on structural carbohydrate fermentation. Neutral detergent residues of leaf and stem alfalfa tissues were incubated inpurifed cellulase and filtrates fractionated using size-exclusion chromatography on Sepharose CL-6B. One major absorbance peak (As20) was observed from these fractions. The fraction containing the absorbance peak (A320) inhibited in vitro digestion of structural carbohydrates by rumen microorganisms. Contents of this CL-6B peak were further fractionated on Sephadex LH-20. In vitro incubations with the one major peak from LH-20 also inhibited digestion of structural carbohydrates. It is not known whether phenolic-portions of the complexes were responsible for fiber digestion inhibitionor if carbohydrate-portions were responsible for the observed effect.